March
8
Lab # 8 Gel Electrophoresis/PCR results 3/8/19
Abel Thomas
3/8/19
purpose: The purpose of today’s lab work was to examine the work of the PCR that was added last week. We did this through gel electrophoresis.
Materials:
Agarose Gel
Control sample
Treatment sample
Micropippeter
Electrophoresis container
Procedure:
- Take samples, both control and treatment from the front of the room
- Bring back to desk and get micropippeter set for 10 uL
- Make sure agarose gel is in the electrophoresis container
- Micropippete the samples into the wells, making sure the wells are on the negative side
- Set the machine to 100V for 30 minutes and wait
- Observe the results
Conclusion/Future Goals: Our DNA was shown to have a lot of sample in it thanks to the PCR. That means we had a very concentrated amount of DNA in that specific region.This will help us when we send off our DNA for sequencing to see what type of organisms were in there. After that we will be able to fully complete our posters.
Storage: We made sure to grab all of our belongings and also made sure that the machine was running properly when we left.