November 16

Lab #13: Ciliate Isolation and Characterization

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Objective: The objective of this lab is to enhance our understanding of ciliates and how they are classified to characterize the ciliates found in our soil sample.

Purpose: The purpose of this lab is to find, identify and classify ciliates from our soil samples. We will then determine our soil type by calculating % of sand, silt, and clay within our sample.

Procedure:

  1. Retrieve the Falcon tube that corresponds to your appropriate identifier.
  2. Determine which layer is silt, sand, and clay.
  3. Using a ruler, calculate the percent of silt, sand, and clay in the soil sample by measuring each layer in centimeters.
  4. Using the chart provided, determine the soil type of the soil sample. Record your results.
  5. Retrieve your non-flooded plate. Create a thin layer used to examine organisms by pushing the soil from the non-flooded plate to one side of the petri dish.
  6. Place the soil sample under a dissecting microscope and examine for any movement.
  7. If any moving organisms is found, transfer them to a concavity slide and place on a compound microscope.
  8. Use a compound microscope to find and identify the organisms.
  9.  Clean work station before leaving.

Data:

Measured Layer (cm) % of Layer
Sand 5.2 32.5%
Silt 2.9 18.1%
Clay 7.9 49.4%

Soil Type: sandy clay

Observations: 

Conclusion:  In conclusion, I was finally able to discover a culture of ciliates within my soil AFTER rehydrating the sample. I found that the ciliate has characteristics and features that would be found in the class Litostomatea.

Storage: The non-flooded plates of soil were stored within the correct lab section. Falcon tubes were returned to the test tube rack. All microscopes were covered and put away and slides were washed and left to dry.

Future Goals: I plan to use the information found on ciliates to help classify the one found today  and use this information in my final presentation.

 

August 31

LAB 2: Ciliates Challenge

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Objectives: 

  • Learn how to use the dissecting microscope correctly
  • Learn to observe and characterize ciliates under dissecting microscope

Purpose: The purpose of this experiment is to learn how to use a dissecting microscope to examine and identify unknown ciliates. We will then use observations to describe characteristics and compare them to drawings.

Procedure:

  1. Obtain a clean well plate.
  2. Using 6 pipettes, place each of the unknown samples into separate wells. Be sure to label each well with the corresponding sample.
  3. Observe each of the samples under a dissecting microscope. Adjust if needed to obtain a clear image. Record characteristics of each to fill in the following table.
  4. Compare observations with the information provided on ciliates in order to identify each.

Data/Observations:

Unknown # Shape Size Movement Location Characteristics
1 Short, rod-like, round Tiny Fast Dispersed Change in direction easily
2 Round Tiny Fast; frequent twirling Grouped; dispersed Constant twirling motion
3 Rod-like Small Fast; glides like a snake grouped Faster than 1 and 2
4 Flat Medium Slow; glides grouped/dispersed Tapered off body
5 Skinny rod-like Medium to large Slower; changes in direction Grouped Very malleable body
6 Long thick body Large Swimming motion Dispersed Constantly moving

Sketches: submitted seperate

Storage:

  1. Clean table area and cover and store dissecting microscope
  2. Bleach and rinse well plate thoroughly. Let dry.

Conclusion: 

In conclusion, by observing and characterizing different types of ciliates through the use of dissecting microscopes, we were able to identify ciliates by their physical traits and movements in an attempt to understand and explain the functions of these structures.