Lab 14
Bassam Ballout
Lab 14
April 18, 2019
Aim:
The aim of this lab was to finalize our abstract and fix all comments given to us by Dr.Adair on our poster so we can finalize them and have them ready to be presented.
Procedures:
- Vote on the Cilicure logo (D) and decide if you want a shirt, sticker etc…
- Collect, revise, and add all soil metadata into cilicure class spreadsheet for saving
- We also relabeled our soil bag due to the fact that we had positive PCR results for our sample.
- Use the rest of lab to re-edit poster and fix all given comments and finalize abstract.
Data:
Metadata:
Poster Title:
The Observation of Biodiversity on the Baylor Campus through Metabarcoding
Abstract:
Soil biodiversity plays an integral role within the soil microenvironment as well as the sustainability of the macro-environment. As a field experiment, soil was collected and analyzed from the rhizosphere of Quercus macrocarpa for metabarcoding using the silica bead DNA extraction method. Using this protocol for DNA extraction, a positive PCR product with relatively pure eDNA confirms the presence of soil microorganisms, possibly ciliates. After collection, extraction, purification, gel electrophoresis, amplification of DNA using PCR, and gel electrophoresis of the amplified DNA, next generation sequencing and analysis are ready to be conducted by quantifying the diversity present through the QIIME2 application and basic bioinformatics tools. By quantifying the microorganisms present, we can better learn and understand the optimum environments for ciliates and other microorganisms for future application. Further testing is necessary to better understand the extent of biodiversity present in the soil of the rhizosphere on Baylor campus. Bioinformatics analysis will be further conducted in regard to quantifying and characterize qualitatively the importance of biodiversity in our environment.
Poster:
Conclusion:
Our poster and abstract have been finalized. All given comments on our poster were edited and fixed. Our abstract is composed of the entire groups multiple abstracts combined into one.
Further steps:
We expect to have our finalized poster ready for the CURES event so that we can present our findings.