Revised Lab 14 4/25/19
Date of Experiment: April 25, 2019
Objectives:
- Develop an abstract for the scientific poster
- Finalize metadata spreadsheet and poster presentations
- Determine sample for Illumina sequencing
Purpose: Determine which sample will be sequenced based on compiled metadata and learn the components of an abstract
Materials:
- Abstract
- Scientific Poster
- Frozen soil samples
- Experimental sample
- Illumina sequencing
Methods:
- Choose candidate samples based on suitable metadata
- Complete DNA extraction and send for Illumina sequencing
- Develop an abstract for the poster based on methods, procedures, results, and the conclusion
- Submit to class Box file
Observations:
Soil ID: SS25_6SP19
Soil Bag Label: BSQS
GPS Location: -97.1135, 31.5491
Tree Species: Pinus taeda
BHD: 70 cm
Soil Texture: Silt loam
Extraction Method: Chelex
DNA Concentration: 112.1 ng/uL
PCR: Positive
Results:
Samples considered for sequencing either underwent Chelex extraction, or followed the PowerSoil protocol. While an abstract follows the pattern of a scientific poster or research paper, it merely summarizes the scientific findings into few, concise sentences.
Conclusion:
The sample sent for sequencing will be compared to those in a genetic database. Here, the relatedness of organisms can be seen among those that have already been analyzed. Developing an abstract is useful for summarizing the overall motives and procedure for conducting a specific experiment.