Objectives:

The objective of this lab was to be able to have large sequences of DNA from the samples that we had from a previous week. The other object was to talk about the poster that will be made in order to present the results of the experiment we conducted.

Purpose:

The purpose of this experiment was to be able to pipet our sample and the ladder into the different wells. Then to be able to conduct electricity through in in order to be able to activate the DNA sequences that exist within our DNA samples.

Procedure:

1. Take you agrose gel and set it up
2. Remove from cast
3. Place it in the electrophoresis box
4. Take the previously created sample and ladder
5. Pipette 5ul of the ladder
6. Place in one of your wells
7. Pipet 10ul of your sample
8. Place it into a separate well
9. Record the order in which the samples and ladder are places
10. Connect to the remaining pieces of the electrophoresis ox
11. Let electricity run through it at 100 for 20 minutes
12. Record and picture the results

Data and Observations:

What was observed from running new gels was that two of the samples that were taken from the soil procedure and one of the samples that was taken from the Chelex procedure were able to produce large sequencing. This could be due to how the following procedures were preformed. Most samples di not create a large enough sequence.

Storage:

Given that not much was done for our experiment, there was not much to store. The only things that had to be stored were the things that related to the agrose gel. We turned in the cast that was used to make out gel. The other thing that was stores were the test tubes which contained our samples and the ladder. In order to store them they were simply returned to the racks.

Conclusion:

In conclusion after observing the different gels and how big of a DNA sequence it contained. The three samples that were able to have enough to sequence were 2 that were done y soil and one that was done by Chelex. My sample did not contain a big enough strand. This could be due to the different procedures and the concentration of ciliates that were found in the original sample.

Future observations:

For future observations we can see tat the best results were gotten through following the soil procedure. This can tell us in which procedure most DNA is able to be extracted. Given how big a DNA sequence is can also tell us the different type od environment the ciliates lived in, this will give us information to assume the environment in which they thrive. This can be useful in the furture to continue experimenting on ciliated and testing out the places where we believe ciliated are more likely to thrive, therefore giving us the largest DNA sequence.