Lab 8: PCR results; Scientific Poster Design
Lab 8: PCR results; Scientific Poster Design
The purpose and objective:
The purpose of this lab is to run the DNA sample through gel electrophoresis and analyze the image. Also, we take the midterm exam and write the draft of poster.
Materials: kb ladder, DNA sample, agarose gel. Gel electrophoresis, voltage machine, micropippetor.
Procedures:
- Add 5μl of 1kb ladder in well #1;
- Add 10μl of control prepared sample in well #7;
- Add 10μl of prepared DNA sample in well #8;
- Run the gel at 100V for 30 minutes;
- Get the image of gels.
Data:
Conclusion and the future goal:
In today’s lab, we analysis our DNA sample to see if enable to perform PCR. From the image, we could see the clear V4 band that indicates we have a high-quality DNA sample. Also, we could learn that the amplified DNA showed approximately 400-500bp. Moreover, we wrote the poster draft. In the future, I hope that we could finish our poster successfully and learn more about the diversity of ciliates in the soil ecosystem.