Lab 8: PCR and Poster Design
3/8/19
Objectives/Goals: The objectives of this lab was to run the DNA samples using gel electrophoresis and get a rough draft of our scientific poster design. The goals were to decide whether the DNA sample was adequate enough to send for PCR and learn about the components of a scientific poster.
Materials: Gel electrophoresis apparatus, experimental and control DNA samples, DNA ladder, micropipettor
Procedure:
- Obtain 1.5% agarose gel
- Load PCR sample and control into gel
- Load kb ladder (10 µl) in gel
- Run gel at 100V for 30 minutes
- View results of gel with gel imagery
Data:
Results: A high concentration with a strong degree of brightness was shown from running the gels, which indicates that there was an adequate amount of DNA in the sample.
Storage: The gels were left to be disposed of, the micropipettes were hung up, and the tabletop was wiped down.
Conclusion/Future Goals: Overall, the DNA shown from the gel electrophoresis was concentrated and a design for the scientific poster was made into a rough draft. In the future, I hope to extract an even purer source of DNA to be sequenced through the PCR technique.