3/8/19

Objectives/Goals: The objectives of this lab was to run the DNA samples using gel electrophoresis and get a rough draft of our scientific poster design. The goals were to decide whether the DNA sample was adequate enough to send for PCR and learn about the components of a scientific poster.

Materials:  Gel electrophoresis apparatus, experimental and control DNA samples, DNA ladder, micropipettor

Procedure:

1. Obtain 1.5% agarose gel
2. Load PCR sample and control into gel
3. Load kb ladder (10 µl) in gel
4. Run gel at 100V for 30 minutes
5. View results of gel with gel imagery

Data:

Results: A high concentration with a strong degree of brightness was shown from running the gels, which indicates that there was an adequate amount of DNA in the sample.

Storage: The gels were left to be disposed of, the micropipettes were hung up, and the tabletop was wiped down.

Conclusion/Future Goals: Overall, the DNA shown from the gel electrophoresis was concentrated and a design for the scientific poster was made into a rough draft. In the future, I hope to extract an even purer source of DNA to be sequenced through the PCR technique.