Lab #14 Final Abstract and Poster 4/26/19
Abel Thomas
4/26/19
Purpose/Objective:
The purpose of this lab was to label and understand our metadata along with correcting our poster and our perfecting the final abstract for the poster.
Procedure:
- Vote on a logo
- Finish total metadata for the class
- Revise poster and the abstract
Metadata:
| Bassam Ballout, Paul Nemer, Abel Thomas | 21 | 7 | BNT21_7Sp19 | -97.1147539, 31.5489044 | Quercus macrocarpa | 71.34 | 6 | Clay Loam | Silica Bead | 722.3 | 10 | + | Bassam Ballout BNT21_7Sp19 |
Title:
The Observation of Biodiversity on the Baylor Campus through Metabarcoding
Abstract:
Soil biodiversity plays an integral role within the soil microenvironment as well as the sustainability of the macroenvironment. As a field experiment, soil was collected and analyzed from the rhizosphere of Quercus macrocarpa for metabarcoding using the silica bead DNA extraction method. Using this protocol for DNA extraction, a positive PCR product with relatively pure eDNA confirms the presence of soil microorganisms, possibly ciliates. After collection, extraction, purification, gel electrophoresis, amplification of DNA using PCR, and gel electrophoresis of the amplified DNA, next generation sequencing and analysis are ready to be conducted by quantifying the diversity present through the QIIME2 application and basic bioinformatics tools. By quantifying the microorganisms present, we can better learn and understand the optimum environments for ciliates and other microorganisms for future application. Further testing is necessary to better understand the extent of biodiversity present in the soil of the rhizosphere on Baylor campus. Bioinformatics analysis will be further conducted in regard to quantifying and characterizing qualitatively the importance of biodiversity in the environment.
Poster:
Storage: After inputting the metadata, we went up to the computer lab and fixed up our poster and our abstract to be submitted into Box.
Conclusion/Future Goals: In lab we were able to complete our metadata for our entire class. And then using the computer lab we were able to complete and fix our errors in our abstract and our posters. With this information we would be able to send our information to a lab and eventually use QIIME to analyze it.