April
19
Lab 13: Soil eDNA Metabarcoding 4/19/2019
Objective:
The objective of today’s lab is to analyze the eDNA sequenced last year
Purpose:
The purpose of today’s lab is to analyze the eDNA using qiime2 to practice
Procedure:
- Check if ITS removed qiime by typing source activate qiime2-2019.1
- Download the box file on to the mac
- Import the sequences into qiime as a .qza file
- Demultiplex the sequences
- Denoise the sequences using DADA2
- Create a feature table
- Create a phylogenic tree
- Create a taxa bar plot
Conclusion:
It was successful, even if it took really long for the computer to process.
Future Goals:
I hope you get better, have a nice easter, and to use this info later.