April 19

Lab 13: Soil eDNA Metabarcoding 4/19/2019

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Objective:

The objective of today’s lab is to analyze the eDNA sequenced last year

Purpose:

The purpose of today’s lab is to analyze the eDNA using qiime2 to practice

Procedure:

  1. Check if ITS removed qiime by typing source activate qiime2-2019.1
  2. Download the box file on to the mac
  3. Import the sequences into qiime as a .qza file
  4. Demultiplex the sequences
  5. Denoise the sequences using DADA2
  6. Create a feature table
  7. Create a phylogenic tree
  8. Create a taxa bar plot

Conclusion:

It was successful, even if it took really long for the computer to process.

Future Goals:

I hope you get better, have a nice easter, and to use this info later.


Posted April 19, 2019 by anthony_capili1 in category Anthony Capili-34

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