Objective and Rationale

The main purpose for this weeks lab meeting was to continue practicing our use of Qiime2 and learning how to use the macOS terminal for genome analysis. By going through the procedure given to us by the TA, we were able to further familiarize ourselves with the process of using qiime2.

Methods

Students who used school computers had to follow the procedure to redownload Qiime to the school computer, and the students with Mac laptops activated Qiime2. We then went to the Qiime website and followed the specific instructions from the online tutorial using data from the website. Our TA gave us a word document list of things to copy and paste into the terminal until we obtained the .qzv files needed for viewing and analysis. Specific instructions were given by the website and by our TA. If problems arose, we would have to backtrack our steps until we finished.

Results

Everyone was able to successfully obtain readings from the Qiime2 by following the steps on the word document. Once we got the .qzv file, we would drag and drop them into the Qiime2 view website in order to see these charts.

Conclusion and Future Goals

This lab went successfully except for a few issues such as having to redownload Qiime2 and having to redo the procedure of the tutorial a few times just because of miscommunication in the lab. Over the course of the next few weeks of CILI-Cure, we will be furthering our knowledge of bioinformatics by practicing more with Qiime2. I look forward to learning more about Qiime2 and practicing with the macOS terminal.

Storage

Students with macs have Qiime2 on their laptops and other students will use the school computers.