Objective:

The objective of today’s lab is to practice qiime 2 through the moving pictures tutorial

Purpose:

The purpose of today’s lab is to learn how to use qiime 2 in order to use it for our own purposes.

Procedure:

1. Ensure conda and qiime 2 is installed in the terminal this can be done by typing conda update conda (to check conda) and source activate qiime2-2019.1
2. Make a new directory named “qiime2-moving-pictures-tutorial” using the mkdir command
3. Change to that directory using the cd command
4. Using the wget command get the sample metadata from the qiime website
   1.  wget \
      -O “sample-metadata.tsv” \
      “https://data.qiime2.org/2019.1/tutorials/moving-pictures/sample_metadata.tsv”
5. Make a new directory named “emp-single-end-sequences”
6. Using the wget command get the sample barcodes and sequences
   1.  wget \
      -O “emp-single-end-sequences/barcodes.fastq.gz” \
      “https://data.qiime2.org/2019.1/tutorials/moving-pictures/emp-single-end-sequences/barcodes.fastq.gz”
   2. wget \
      -O “emp-single-end-sequences/sequences.fastq.gz” \
      “https://data.qiime2.org/2019.1/tutorials/moving-pictures/emp-single-end-sequences/sequences.fastq.gz”
7. Import

Conclusion:

Future Goals: