Aynsley Gaspard

4-11-19

BIO 1106

Lab 12

Purpose:

The purpose of the lab was to introduce ourselves to NGS, QIIME2, and analyzing sequences and running them in Terminal. This is all in pursuit of mastering Metbarcoding.

Procedure:

1. First, we needed to lead QIIME2 on our Terminal.
2. We then downloaded and copied Moving pictures into our files and Terminal.
3. After that, we downloaded a sample metadata file.
4. We then copied the sequencing command into Terminal.
5. Then imported the tools command into Terminal.
6. Next, we moved on to Demultiplexing.
7. We copied the qza command from that into Terminal.
8. Then the qzv command.
9. This brought us to the visualization step.
10. We copied the DADA2 denoise and table qza command into Terminal.
11. We then went to the visualizing section of QIIME2 and drug the table into the box that pops up.
12. We then moved on to taxonomic analysis and copied the taxonomy qzv  and bar ploys qzv commands into Terminal.
13. This file was hen drug to the box in QIIME2 again to visualize.
14. This then showed us all the different taxa among our sample metadata.

Data:

Our sample data was shown in the QIIME2 visualization section.

Future Steps:

With this, we can determine the different species found in the sample data. Also, we can use the process we learned to sequence our own data from our soil collection.

Conclusion:

Overall, we learned how to use QIIME2 and Terminal to metabarcode data and determine different taxa among the species found.