Chandler Opara

April 4, 2019

QIIME 2 Installation and Terminal Practice

Objectives:

The objective of this lab was to use the Apple Mac OSX terminal to install the QIIME program. The purpose of this lab was to familiarize ourselves with the different type of codes used in the terminal. This program will allow use analyze the data collected from our samples in the following weeks.

Procedures

Installing QIIME 2

1. Open Terminal on Mac by using the launch pad
2. Practice using the terminal by stating commands
3. Download Miniconda(64-bit (pkg installer) ) to begin the process of download qiime
4. Close terminal and then reopen the terminal
5. Once downloaded, type “conda update conda” into the command
6. Type “y” to proceed with the process
7. Type “conda install wget” into the command
8. Type “y” to proceed with the process
9. Copy and paste the link from the webpage called “Natively installing QIIME 2” into the command
10. Activate the QIIME 2 by typing “activate qiime2-2019.1” into the command
11. Type: qiime –help in the terminal to test the installation

Data

Common Command Used

“Is”: list applications

“cd~/Documents”: to change the directory

“mv” command : can be used to move a  file

Conclusion/Future Goals:

In this lab, we were becoming familiar with the QIIME 2 Program to help us better understand how to analyze the data given to us. Understanding the analysis of our data will help us determine the various kinds of microorganisms within the samples. This will allow us to gather more knowledge about the diversity of the community and conclude how the eDNA impact the surrounding area. In the future, I’m curious to see what new information the reads will give us and how we will interpret them. I’m also excited to see how the use of QIIME 2 will contribute to the conclusions being made.