Objective:

The objective of this lab was to become familiar with Next Generation Sequencing and Metabarcoding through the Illumina Card Challenge, to display our knowledge through completing the QTM, and to become introduced to Cyverse.

Purpose:

The purpose of this lab was to prepare our group for the next portion of labs focused on sequencing of DNA and metabarcoding. This accomplished through the QTM and card challenge.

Procedure:

1. The powerpoint will be presented with an introduction over the material for the lab.
2. Complete the Illumina Card Challenge.
3. Complete the QTM.
4. Turn in ziplock bag containing the parts to the challenge and the QTM before leaving.

Data/Observations:

There was no data or observation gathered during this lab.

Conclusion:

In conclusion, this lab  proved to be very informational and beneficial in gaining new information about the process of sequencing. It included steps of tagmentation, bridge amplification, cluster formation, and chain termination within sequencing. We also created Cyverse accounts and learned about the great opportunities available to our group within Cloud Computing.

Storage:

The computers were turned off and all QTMs and ziplock bags with turned in.

Future Goals:

In the future, I hope to conduct metabarcoding and sequencing with the DNA gathered from the rhizosphere of one of Baylor’s trees. I also hope to present the information in our updated poster to show the knowledge gained throughout this semester.