Sydney Ortenberg

March 8, 2019

Lab 8: PCR Results

Objective/Purpose:

The objective of this lab was to perform PCR gel electrophoresis and begin planning out our poster. The purpose of this lab was to see our DNA samples under the PCR electrophoresis and determine whether or not our sample meets the qualifications to be sequenced.

Procedures

It is important to Wear gloves during this lab because there are caustic items.

1. Obtain the 1.5% agarose electrophoresis gel prepared last lab.
2. Obtain the 1 kb ladder sample and the control and treatment DNA samples that were prepared last lab.
3. Add 5ul of the 1kb ladder into the first lane.
4. Add 10ul of each sample into their designated lanes.
5. Run the gel for 30 minutes at 100V.
6. Dr. Adair took the gels to the secondary lab and analyzed them with the MCB C305.

Data:

Storage:

The gels were left in the fridge at 4 degrees Celcius. All equipment was disposed of and cleaned up.

Conclusion and Future Steps:

In conclusion, this lab allowed us to perform PCR electrophoresis in order to analyze our DNA to see if it is worthy of being sequenced by a company. If it is up to standard, it will be sent off to be sequenced and then shared with the whole scientific community. The midterm quiz allowed us to reflect everything we have learned this year and test to see whether we have paid attention to details and grasped everything we have done. The brainstorming of our poster allowed us to start thinking about how we are going to present everything and have an overall idea of what we are trying to accomplish. I am excited to analyze my DNA and see whether it can be sequenced or not. I am also looking forward to making our poster because I think it will be a unique and creative way to present all of our findings.