March 7

CiliCure Lab 8

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Aynsley Gaspard

3-7-19

BIO 1106

Lab 8

Purpose:

The purpose of this lab was to run our DNA sample and to get an image of it.

Procedure:

  1. Set up 1.5% gel
  2. Load 5ul of PCR sample gel in 4th row  (I did the 4th row for my group’s sample).
  3. Load 10ul of 1 kb ladder to 1.5% gel in 5th row  (I did the 5th row for my group’s control).
  4. Run at 100V for 30 mins
  5. Take image of the final DNA concentration after it is run.
  6. We went up to the A305 to take Midterm and go over poster design.

Results:

This image shows our groups DNA after it was run. In the 4th row, it shows a faint concentration of DNA which my partner and I’s DNA sample (group 7). Row 2 and 3 are the DNA sample and the control of group 6.

Future Goals:

Future goals for this lab are to sequence the DNA we see in the image above and to discover the genus species of the varying amounts of ciliates. This will help with the discovery of bacteria and diseases that could affect the tress around Baylor’s campus.


Posted March 7, 2019 by aynsley_gaspard1 in category Aynsley Gaspard-33

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