2/28/19

Objective:

The objective of today’s lab was to amplify our DNA using PCR.

Purpose:

The purpose of today’s lab was develop PCR skills for future research and to have our DNA amplified to receive our soil DNA data.

Experimental Procedures:

• We received our ice container which was keeping all of our ingredients cool.
• First, we performed a 1:1 dilution on our DNA.
• We filled a tube with 5 microliters of soil DNA and 5 microliters of water.
• From that tube, we withdrew 1 microliter of solution and placed it in the tube with 12.5 microliters of 2x master mix.
• Next, we added 1 microliter of primer at a 10 micromolar concentration to the tube of solution.
• Finally, we added 10.5 microliters of water to the solution. Which gave us a total of 25 microliters.
• We labeled this tube T for Treatment.
• Next, we had another tube filled with 12.5 microliters of 2x master mix.
• We added 1 microliter of primer at a 10 micromolar concentration and 11.5 microliters of water to the tube.
• However, no DNA was added because this will be our control tube.
• We labeled this tube C for Control.

Data/Observation:

We did not observe any data this week. We only prepared for the PCR test. Soon, we will run the PCR and see the results from our tests.

Storage:

We put the Control and Treatment tubes on the rack and put the DNA back in the cooler.

Future Steps:

In the future, we will interpret our results from the PCR and present our findings at the symposium.