Objective

The objective of this week’s lab was to prepare our DNA sample to go through PCR amplification.

Purpose

The purpose of preparing our DNA sample was so that we can further analyze it through PCR amplification.

Procedure

1. Collect your DNA sample from the freezer, primer, H2O, and 12.5 microliters of Master Mix
2. With a micropipette, transfer 1 microliter of primer into a centrifuge tube and label it “DNA”
3. To the centrifuge tube labeled DNA, add 5 microliters of the DNA sample
4. To the centrifuge tube labeled DNA again, add 6.5 microliters of water
5. Repeat step 2 in a centrifuge tube labeled “control”
6. Transfer 11.5 microliters of water into the centrifuge tube labeled “control”
7. Centrifuge both tubes for about 30 seconds to ensure all liquid has gone to the bottom
8. When finished spinning, transfer the contents of the DNA tube into a green tube with 12.5 microliters of Master Mix with a micropipette and label it “DNA”
9. Do the same as step 8 with the control group and label the green tube as “control”
10. Place both green tubes in freezer for storage until DNA is analyzed at a later date

Cleanup and Storage

Micropipettes were hung on the racks and the tip boxes were placed on the prep area. The DNA sample was placed back in the freezer with the green tubes. The primers and water was put away by the lab assistant. All trash was thrown away and lab area was wiped down.

Future Goals

My future goals would be to learn everything we can about our DNA sample. We weren’t able to see our DNA in the gel after it went through the process of electrophoresis so I would like to learn as much as we can from other data collections.