Abel Thomas

3/1/19

Purpose:

The purpose of this lab is to set up the PCR reaction using our DNA and combining it with the Supermix along with other substances. We also worked on our poster design.

Materials:

gloves

eDNA

primers

Supermix

water that was autoclaved

PCR tube

Falcon Tube

Bleach

Micropippete

Procedure:

1. Put on gloves
2. bleach the table to ensure clean working environment
3. calculate the amount of DNA and water needed to make sure that both the control and treatment solutions end up being 25 uL
4. obtain an ice bath along with an empty tube, the tube containing DNA, and the V4 primer tube
5. obtain 2 tubes that contain the supermix, label which one will be the control and the treatment
6. add 1 uL of primer to both tubes
7. Add 11.5 uL of water to control and 10.12 to the treatment
8. Add 1.38 uL of DNA to treatment tube
9. make sure that both tubes are mixed well

Storage:

We made sure that we cleaned our work area and put our tubes in the ice container to preserve them. We also properly removed the pippetes.

Conclusion/Future Goals:

We were able to set up our DNA for PCR amplification. Next week we can hopefully observe the PCR amplification and then the week after we can send our information to get sequenced and see the DNA that we took from the soil.