2/28/19

Sophia Shaikh

Objectives/Goals: The objectives of this lab were to learn about V4 ribosomal primers, PCR, and scientific poster design. The goals of the lab were to prepare our DNA tube and the control tube with the Master Mix and primer to be run through the PCR process.

Materials: Micropipettor, 2 new tubes, V4 primer, Master Mix, sterile water, small ice tub

Procedure: 

PCR Preparation

1. Complete necessary calculations to perform PCR assay in 25 μl of reaction mixture
2. Put on gloves and wipe down work area with bleach to prevent contamination of DNA sample
3. Obtain materials
4. Perform 1:10 dilution on DNA sample because my group had a large amount of DNA (884.6 ng/μl)
5. Pipet 1 μl of diluted sample into new tube *while changing pipet tip each time*
6. Pipet 12.5 μl Master Mix into tube with diluted sample
7. Pipet 10.5 μl water into tube
8. Repeat in a control tube with all components except DNA sample

Data:

Observations: This lab was not very experimental because we only prepared the tubes for PCR, so there were no observations.

Storage: The PCR tubes were put in the block with all of the other groups, the other tubes were kept on ice in the cooler, and the pipettors were hung back up.