Objective:

The objective of today’s lab is to analyze the recently purified DNA in gel electrophoresis.

Purpose:

The purpose of today’s lab to finally observe the DNA that we have been preparing for weeks.

Procedure:

1. Practice filling wells with 10x buffer liquid
2. Obtain gel and purified DNA
3. Break gel out of its mold and place in gel electrophoresis machine
4. Add the 10µL DNA samples and 5µL DNA mass standards into individual wells
5. Turn on the gel electrophoresis and run 100 volts for 20 minutes
6. Take gel up to a machine to be analyzed

Observations:

The contents of the wells were in this order: Well 2 has Group 1’s DNA, well 4 had the low DNA Mass Standard, well 5 had the high DNA Mass Standard, Well 7 had Group 3’s DNA, and all other wells were used for practice.

Storage:

The gel was given to LA.

Conclusion:

The lab was successful for both groups as both DNA samples showed high amounts of DNA, and don’t have to repurify DNA

Future Goals:

I am excited to do PCR next week and hope for positive results.