Objectives:

The objective of the lab was to be able to add the chelex to out ciliate pellets which contain the DNA sequences. As well as to learn how to build a cast and be able to make the gel like solution for our DNA strands.

Purpose:

The purpose of the experiment was to be able to finish the chelex procedure on the pellets that continued part of the ciliate culture, which had been cultivated two weeks prior. Then we have to make the gel which will contain different plates that ill contain the DNA of our sample. This will be useful because it will isolate the DNA of the sample so it can easily be arranged into a sequence, in order for our ciliates to be described.

Procedure:

-Chelex

1. Use test tubes with the chelex balls
2. Incubate for 30 minutes in 56 degrees Celsius water bath
3. Boil for 8minutes
4. Vortex for 1 minute
5. Centrifuge @16,000 for 3 minutes
6. Pipet the supernatant into a new tube
7. label

-Agrose Gel

1. Place gloves
2. Make gel cast
3. Using a test tube place 36ml of buffer, 4ml DIH2O, .4g of power
4. Stir
5. microwave for 1 minute
6. stir
7. microwave for 1 minute
8. stir
9. add ethidium bromide
10. place in cast
11. once soil ass more buffer
12. place in a zip lock bag
13. label
14. refrigerate

Data and Observations:

During this weeks lab there were not many observations that could be made given that we were only preparing the materials that will help us extract the DNA.

Storage:

In order to store the materials that we used we turned in the materials such as the flask, metal plate, test tube with the supernatant and test tube. The pipet tips and  tubes tube with the chelex pebbles were disposed. The cast for the gel was put in a zip lock bag and put in the fridge.

Conclusion:

In conclusion the work that we did was useful because it will help in future labs to be able to extract and decode the DNA. The procedures that we preformed will help us in the future when we are actually extracting the DNA from out samples.

Future observations:

The procedures that were preformed during lab will help us next week by having pure and easily visible DNA strands.