September
13
Lab #4: Meet Tetrahymena 9/13/18
Goals:
- practice microscopy
- learn to use pipettor
- meet Tetrahymena
- literature research
Procedure:
- Observe the Tetrahymena in the well-plate using the dissecting microscope
- Use the pipettor to get 5 macroliters of the cells and put it on a concavity slide
- Look at the slide through a compound microscope
- Observe it through the 4x, 10x, and 40x and observe the ciliates
Data:
Trials | # of cells in 5 macroliters | Approximate Diameter |
1 (4x) | 100 | about 50 cells across |
2 (10x) | 45 | about 20 cells across |
3 (40x) | 20 | about 8 cells across |
Future Goals:
I am hoping that I can make an experiment to see how microplastics affect soil by using Tetrahymena. I am going to do that by looking at the reproduction rates of the Tetrahymena over a period of 24 hours and see how much the microplastics affect it.