Lab 3: Compound Microscopy
Alexandra Patino
9/7/18
Bio 1105-32
Objective-
The objective of this lab was to see the ciliates on the tree life, correctly use a compound microscope, and use cellulose and stains to observe a specific ciliate.
Materials-
- a compound microscope
- a dissecting microscope
- pipettes
- iodine
- cellulose
- well plates
- cover slides
- 6 ciliates
Procedure-
We were first taught the field of view formula, then calculated the field of view for our own compound microscope. Next we used the dissecting scope to see into the wells to find a ciliate. When we found the ciliate we wanted, we obtained it with a pipette and placed it onto the observing slide to view under the compound microscope. Once under we viewed it on 4x, 10x, and 40x magnification. I then recorded my discoveries in my lab journal and then placed a drop of iodine to better see the organelles of the ciliate.
| diameter (mm) | diameter (μm) | |
| 4x | 4 mm | 4000 μm |
| 10x | 1.6 mm | 1600 μm |
| 40x | .4 mm | 400 μm |
| Ciliate #6 | On slide before Iodine | Iodine |
| Size/Color | Fairly large (about 600 μm), green in color | Same size, but color was different due to the dye |
| Shape | Bulb shaped | Same as without Iodine |
| Movement | A lot of movement, body contrats and extends tail | No movement because it died when I added the Iodine |
Conclusion-
I am able to correctly use a compound microscope and calculate my field of view. I also know how to use iodine and the hanging concavity slide to view my ciliates. I believe ciliate #6 is a Stentor, which I discovered by the end of the lab.