Lab 7: Open Lab
2/23/17
Based on open lab
Purpose: The purpose of lab for me today was to come in and learn what we did in class and complete the QTMs.
Procedure:
- Go through powerpoint with Will.
- Read protocol for agarose gel preparation.
- Answer QTMs.
Results/What did I learn/Conclusion:
Today I was able to catch up from missing lab on Thursday due to being sick. Will and I went through the powerpoint in depth and discussed the procedure my group did in class. I was able to identify what our template and DNA primer we would be using. I did a calculation to see what our final concentration of primer in our stock tube would be. To get my answer, I used the C1V1=C2V2 formula. I reviewed the temperatures and time needed to perform PCR.
Future Steps:
The next step for our lab would be to perform gel electrophoresis after PCR has completed amplifying the DNA of our ciliates and our gel agarose has hardened.