Purpose
The purpose of lab 13 was to run the electrophoresis through 1.8% agarose gel that we made last week. We ran the gel through the reactions that we prepared in the previous lab. While we were waiting for the electrophoresis to finish setting up, our group divided the roles of our poster presentation and we agreed on the design of our poster. Once the electrophoresis was completed, we looked at the gels under UV light to determine if the Chelex protocol was successful.
Procedure:
1. Pour the 1X TAE solution into the gel powerboat so there is a thin layer on the top of the gel

2. Place 5uL drops of the reactions into the wells of the gel

3. Record which reaction is in each well

4. Run the electrophoresis at 100 volts for 30minutes

5. While the electrophoresis is being performed, discuss the poster designer and  what information will go where

6. Assign roles between group members

7. Once the electrophoresis is completed, take the gel upstairs to be analyzed

8. Save the image of the results and record the results for each reaction

9. Clean up lab area
Data and Observations

Reaction Set-Up from right to left 

◦ 21-2e (eDNA)

◦ 21-1e ◦ 21-2+

◦ 21-1+ ◦ 21-2-

◦ 21-1- ◦ Blank well

◦ Ladder
Results

◦ 21-2e had a faint band of results

◦ Both positive controls showed bands of results

◦ Ladder had results

◦ Nothing from the other wells

Presentation  Austin: Results and Conclusion/Future Work

Tim: Methods

Christian: References, abstract and Introduction

Conclusion and Future Work: 

Although we took the time to reperform the Chelex protocol, we were stilled filled with disappointment by our results. We had no significant results or any satisfying amount of DNA. However, we have learned that although the results were negative, they are still significant in the research of the protocols. Continuing into our final weeks of lab, we will now begin the preparation for presenting our results.