Purpose:

The purpose of the lab was to run the gels prepared from the last lab, analyze those results, and begin a working template for the poster presentations as well as setting up the abstract.

Procedure:

1. Remove the rubber ends from the agarose gels made from last week’s lab and place the gels in the gel electrophoresis box containing 1X TAE buffer.
2. Ensure that the gel is covered with the TAE buffer and add 5 µl of the ladder to well 1 using a p20 micropipette.
3. Using a new micropipette tip for each well, add 10 µl to each designated well:
   • Well 2: Group 7  negative
   • Well 3: Group 7  positive
   • Well 4: Group 7 eDNA
   • Well 5: Empty
   • Well 6: Group 8 negative
   • Well 7: Group 8 positive
   • Well 8: Group 8 eDNA
4. Close the electrophoresis box and connect the power sources ensuring that the red cord corresponds to the red input and output sources. Ensure the black cord corresponds to the black input and output sources.
5. Run the gel electrophoresis at 100 volts for at least 30 minutes.
6. Remove the gel and examine the gel under a UV light.
7. Take a picture of the gel and record the results.

Data/Results:

Group 7 and 8 gel results shown in the picture below:

• Wells are arranged from well 1 (most left) to well 8 (most right).
• There were no bands but a faint band in well 4 around 500 bp which was the group 7 eDNA sample

Conclusion:

In conclusion, the procedure did not go as planned because we did not have successful results as we hoped. There had to be some type of error during the experiment because the eDNA had a faint band and the positive control had negative results. Our hopes for the future is to find an even better protocol to obtain desired results. My group also planned out how the poster will look like and details going into the abstract as well as our goals on when to finish everything.