Date of Experiment: 12 April 2018

Experiment Title:Gel Electrophoresis Part 3

Rationale/ Purpose:  The goal of this experiment is to place the samples created in the previous lab into the gel and run the gel. Additionally, we learned about and wrote an abstract for our poseter while the gel was running. After the gel ran for around 30 minutes, we viewed the gel under ultraviolet light.

Procedure: Place the gel created from the previous lab into the box and insert the samples into the wells. The ladder was placed in well 1, – control in well 6, + control in well 7, and eDNA in well 8. Place the lid on the box and run the gel for around 30 minutes. While the gel was running, we learned about creating a poster, wrote an abstract, and designed a poster.

Observation/Data/Results:

Gel under UV Light

There was no band in the negative control or the positive control. There were faint bands in the environmental DNA.

Interpretation/Conclusion/ Next Steps: In this experiment, we were able to see the DNA travel towards the positive side, creating bands based on the size. Our samples did not run as well as we thought. We were able see the bands in the gel, but they were very faint. Also there were no bands in the positive control. In the next lab, we will continue to work on our poster.

Sample Storage: The PCR tubes were labeled 22-6 -, 22-6 +, 22-6 e. The tubes were returned to the Dr. Adair.