Purpose: Perform gel electrophoresis on our cox1 and V4 samples in our agarose gel from the previous lab

Procedure:

• Obtain gel made from previous lab meeting from the freezer
• Pour in 1x TAE stock solution over the gel
• Micropipette 5ul of the ladder into the well 1
• Using a new micropipette tip for each well, add 10 µl to each designated well:
  • Well 2: Positive Control Cox1 solution.
  • Well 3: Negative Control Cox1 solution.
  • Well 4: Environmental Treatment Cox1 solution.
  • Well 5: Positive Control V4 solution.
  • Well 6: Negative Control V4 solution.
  • Well 7: Environmental Treatment V4 solution.
• Set up and run the gel box for 30 min at 100 volts
• After, carefully take out gel and observe under the UV light

Results:

This is our Gel after the 30 minutes at 100 volts.

Our gel under the UV light. Both negative cox1 and v4 solutions gave a positive outcome as well as the ladder, and all the other results were invalid.

Conclusion:

The whole process of running the gel electrophoresis and observing the gel under the UV light went smoothly and gave more desirable results, and the gel was discarded after the lab.