Today we performed gel electrophoresis on our controls and samples with the COX1 and V4 primers.

Procedure:

• Place prepared 1.8% gel in electrophoresis box
• Fill the box with 1x TAE until it covers the gel
• Place each of the samples which we performed PCR on last week in individual wells in the gel
• Load the ladder into one of the empty wells
• Put the lid on and attach the electrodes to the power supply
• Run it on 110V for approximately 30
• Remove the gel
• Image the gel in a ultra violet imager

gel during electrophoresis

Wells from left to right: Ladder, Empty, V4 experimental, V4 positive, V4 negative, COX1 experimental, COX1 positive, COX1 negative.