Task, Rationale, and Purpose: Lab week 11 focused on the analysis of our purified DNA. The task of the lab was to run a gel electrophoresis of our treatment DNA and controls. Secondly, the results of the electrophoresis was analyzed using a UV light and recorded. The purpose or rationale of the experiment is to learn how to analyze and measure base pair length and conclude positive or negative results. These results will be able to discuss the implications of them in relation to how certain protocols performed in DNA extraction and purification.

Gel Electrophoresis:

1. Remove the rubber ends from the agarose gels made from last week’s lab and place the gels in the gel electrophoresis box containing 1X TAE buffer.
2. Ensure that the gel is covered with the TAE buffer and add 5 µl of ladder to well 1 using a p20 micropipette.
3. Using a new micropipette tip for each well, add 10 µl to each designated well:
   1. Well 2: Positive Control Cox1 solution.
   2. Well 3: Negative Control Cox1 solution.
   3. Well 4: Environmental Treatment Cox1 solution.
   4. Well 5: Positive Control V4 solution.
   5. Well 6: Negative Control V4 solution.
   6. Well 7: Environmental Treatment V4 solution.
4. Close the electrophoresis box and connect the power sources ensuring that the red cord corresponds with the red input and output sources. Ensure the black cord corresponds with the black input and output sources.
5. Run the gel electrophoresis at 100 volts for at least 30 minutes.
6. Remove the gel and examine the gel under a UV light.

Data/Results:

Gel prior to running for 30 minutes at 100 volts:

Gel after running for 30 minutes at 100 volts:

UV light results:

Storage: The Agarose gel was disposed in a plastic bag. The gel electrophoresis box was left to dry on table 8.

Conclusion: The results from the gel electrophoresis showed no DNA was present. Having no DNA was expected for all wells except for the positive control. The lack of results from the positive control well is an issue and may have been due to an error with the making of the solutions. For future experiments, the Chelex protocol will be repeated due to the lab having promising results from their lab week 11 experiment.