Purpose 
The pups of this lab was to perform gel electrophoresis on our 4 samples previously collected: ladder, positive control, negative control, and soil sample. However, in order to ensure we properly knew how to perform the insertion of the sample drops into the gel, we practiced using dilution samples containing no PCR. After we interred the samples into the wells in the gel, we peer-edited the rough drafts of our introductions from the pre-lab. We put together our thoughts from each individual rough draft, so we could write a new introduction as a group fro the following lab.
Procedure

1.  Locate gel mold from last week and prepare the loading of the gel with the prepared dilution of 1x TAE and distilled water
2. Remove the comb
3. DNA will be running towards the positive side of the electrode
4. Add 5uL of any practice solution that contains the buffer
5. Transfer the solution to the three left wells and practice carefully depositing the gel
6. Transfer 10 uL of the ladder into one well
7. Transfer 5 uL of the positive control, negative control, and soil sample into a well
8. Begin the electrophoresis through the gel at 100 volts for 30min
9. While electrophoresis takes place, swap introduction from the pre-lab with a group member and revise according to provided rubric
10. Exchange constructive ideas with group members to improve the introduction
11. If there is enough time, view gel under UV light

Data and Observations 

Organization of Wells:

No errors were made in the deposition of the samples into the wells

Wells from left to right contained 3 consecutive practice samples

From left to right after the practice samples are the 10uL of ladder, soil sample, positive control, then negative control

Our group did not have enough time to view the results of the electrophoresis

Conclusion and Future Steps 
From this lab, the group was introduced and became familiar with dilutions involving concentrations of TAE. We successfully placed the entire samples in all the wells, so that the electrophoresis could be conducted. We well examine the results in the next lab under UV light; UV light is a key component in the examination of amplified DNA. If the electrophoresis was successful, we will continue by sequencing the DNA and discover matches in the genome of ciliates. If the electrophoresis is negative, we will have to start eh Ludox centrifugation stage  and most likely make some changes to the protocol, so we can receive a positive result.