Title: Gel Electrophoresis 3/1/2018

Materials:

• Gel with cell wells
• electrophoresis apparatus
• Loading Buffer

Procedure:

• Add 30 mL of 10X TAE to 270 mL DI water.
• Add gel to gel electrophoresis chamber and pour in the buffer.
• Add 25 μL of water and 5 μL of Loading Buffer into a tube and mix thoroughly.
• Practice adding 5 μL of the Buffer to the cell wells.
• Add 5 μL of Ladder to a cell well (for comparison)
• Add 5 μL of dye to PCR products (+/- control, and experiment sample)
• Add 10 μL of each PCR product to their designated wells.
• Put the lid on the chamber with the wires connected.
• Set the power supply to 110 volts. Run for 30 minutes.
• Remove the gel and scan with UV imaging to search for DNA.

Results:

We were not able to complete the protocol so there are no results to report.

Mistakes:

We did not get any PCR products, which indicates that there was a mistake or error in the PCR procedure. It is possible that the genes were not amplified enough to read the DNA.

Conclusion:

The next step would be to check for DNA using the UV light. If the DNA is positive, then we will begin attempting to classify the information and use the DNA to identify ciliates. Identifying DNA is important because it will enable us to figure out the type of cilitates we are dealing with.

Stored: Our cell wells are stored in the lab room. It is labelled KCLMCK.