This day’s task was to take our samples of isolated cellular material and prepare them for DNA extraction.  The first step was to wash the samples sever time in order to remove any non cellar material that may have still been present.  After this we extracted the DNA from the cells through a series of denaturing agents, filters, and centrifugations.  The exact steps followed are recorded in the images below.  Steps 1 and 2 were carried out 3 times before we moved on to step 3.  This was to ensure no impurities made it into our final sample.

After step 2 we extracted 20 µl from our sample and stained it with 20 µl of iodine.  The below images are of 2 µl samples of the iodine stained cells.

From counting the cells in these samples we got an average of 10,000 cells per 200 µl

Now that our DNA has been extracted and isolated, the next step is to complete PCR.