Purpose: The purpose is to extract ciliates from a soil/water sample

Procedure: 

1. Add 8ml Ludox HS 40 to a 15ml conical tube
2. Quickly add (inject) 2ml of liquid from the soil samples in the jars into the Ludox using a p1000.
   1. Cut the tip to avoid getting soil stuck and avoid picking up grass and sticks from the sample
3. Carefully add (layer) 2ml of distilled water to the top of the mixture. Don’t mix.
4. Centrifuge in a swinging bucket rotor for 15 min at 4300 x g
   1. Make sure your tubes are equal mass before loading the centrifuge. Add water to balance.
5. Carefully remove the tube and use a pipette to remove the cell layer (visible under the water layer)
6. Transfer to a clean microfuge tube.
7. Transfer 10 microliter drips to a concavity slide using a micropipetter and count the cells using a microscope and record observations.
8. Label your tubes
9. Spin the cells in a microfuge at 12000xg for 1min
10. Remove the supernatant and leave the pellet and store.

Data:

ludox tube mas: 21.3g

Conclusion:

In conclusion, there was 1 cell found in my slide sample and the tubes were labeled SAM.