Purpose:

• In this lab, the objective was to begin our investigation of whether or not the use of a fertilizer agent NH4Cl has an effect on the Tetrahymena population.

Procedure

1. Gather a 24-plated well and add 450 microliters of PPT media onto the top 3 wells
2. The researchers will then add 50 microliters of the Tetrahymena stock culture and mix it. This will be the first dilution made(1:10)
3. More serial dilutions will be created by continuing this along those three wells. After each of the dilutions have been formed, take 10 microliter drops from each well and count the number of cells in each of the drops.
4. Once the average cell count is obtained, in each well 950 microliters of PPT media was added where 3 of the wells would be the control and another 3 would be the treatment group.
5. In the 3 control group wells, 20 microliters of water would be added and mixed whereas in the treatment group 20 microliters of NH4Cl would be added.
6. Once a 24 hour time period passes then 10 microliters of iodine is placed in the control and treatment wells.

Results/Findings

Conculsion

• This experiment allowed the researchers to better understand the kind of impact that NH4Cl has on the population of Tetrahymena. Also, this effect of the Tetrahymena can lead pave the road for future studies to further investigate how this fertilizer with NH4Cl can affect this model organism.