Purpose:

• In this lab, the objective was to determine how to calculate the number of cells through the use of serial dilutions by finding the concentration.

Procedure.

1. The researchers gathered the materials needed for this lab such as a compound microscope, a concavity slide, PPT, and micro pipettes.
2. First, 450 microliters of PPT was added to the tube and 50 microliters of the Tetrahymena culture was also added to this tube.
3. The contents were then mixed and 10 microliters of the solution was then transferred using a micropipette and the researchers then counted the number of cells present in that drop.
4. The researhcer will then make a 1:10 dilution by using 90 microliters of PPT and added 10 microliters of the Tetrahymena solution from the previous solution.
5. This process was repeated until the cells were easily counted thus allowing the researchers to determine the concentration of the Tetrahymena culture.

Conclusion

• Overall the lab allowed the researchers to develop the skill of creating serial dilutions in order to effectively count cells and use micropipettes.