November
30
Lab 12–Isolation and Observation of Ciliates from Soil Samples–9 November 2017
Purpose:
The purpose of this experiment was to use a Falcon tube to classify our soil. We also observed our soil samples in attempt to locate live ciliates, isolate them, and extract them. This lab allowed us to gain more information on our soil that will be necessary for future use.
Procedure:
- Measure cm of sand, clay, and silt in the Falcon tube.
- Apply measurements on soil identification chart to determine clay type.
- Observe soil sample under dissecting microscope, and attempt to locate ciliates.
- If any ciliates are located, draw up individual ciliates using a micro pipette.
- Place ciliate extraction in a well containing 750 microliters of protozoa pellet.
- Isolate one ciliate if applicable and place it on a concavity slide. Record your ciliate with pictures.
Data:
Soil Type | Clay |
Soil Color | Dark Brown |
% Water | 19% |
pH | 7 |
% Sand | 43% |
% Silt | 52% |
% Clay | 5% |
Clear Separation | Yes |
I was not able to identify any ciliates in my soil sample.
Conclusion:
Although I was not able to identify and isolate any ciliates, I was able to identify my soil type.