November 27

Lab experiment #13 (11/16/17)

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Purpose: The purpose of this experiment was to discover and try to identify the ciliates found in the previous lab.

Procedure:

  1. Bring the well plate with your ciliate culture to your desk.
    1. If you did not create a well plate because you couldn’t find ciliates in your soil sample, create a well plate using Dr. Adair’s ciliate sample.
  2. Examine the well plate under a dissecting microscope to find more ciliates.
  3. After finding ciliates in your well plate, pipette 10 microliters of the sample on to a slide.
  4. Use a compound microscope to find ciliates on your slide.
  5. Take photos of your ciliates.
    1. If you can’t take photos of the ciliates because they are too fast, pipette about 3 microliters of methylcellulose on your sample.

Results:

I found three ciliates in my soil sample, but I could not capture and culture them. I ended up using Dr. Adair’s sample, and I was able to find more ciliates. 

Conclusion: Overall, this experiment was an effective way to observe ciliates in different types of soil. Although I wasn’t able to capture ciliates from my soil, it was interesting to see the ciliates in Dr. Adair’s soil sample.


Posted November 27, 2017 by ally_kang1 in category Ally Kang, Bio 1105-34

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