October
7
Lab Experiment #5 (9/21/17)
Purpose: The purpose of this experiment is to get comfortable with serial dilutions.
Procedure:
- Label 5 microcentrifuge tubes from 0 to -4.
- Add 10uL of the sample into the first tube that is labeled 0.
- Add 90uL of media to the tubes labeled -1 to -4. Do not add to the tube labeled 0.
- Add 10uL to the tube labeled -1. Vortex the tube for 10 seconds to mix the sample.
- Add 10uL from the tube labeled -1 to the -2 tube. Vortex the tube for 10 seconds.
- Repeat step 5 for the rest of the tubes by adding 10uL from the previous solution into the next tube. Then vortex.
- Place 5uL of each sample onto a concavity slide.
- Place the slide under a microscope and observe.
- Observe and count how many cells are in each dilution sample. Record observations.
Results:
Conclusion: Overall, students that participated in this lab were able to learn more about serial dilutions. I personally thought that this lab was really interesting to do because we got to see how cells react when living in the same samples, but different dilutions.