Purpose: The purpose of this experiment is to get comfortable with serial dilutions.

Procedure:

1. Label 5 microcentrifuge tubes from 0 to -4.
2. Add 10uL of the sample into the first tube that is labeled 0.
3. Add 90uL of media to the tubes labeled -1 to -4. Do not add to the tube labeled 0.
4. Add 10uL to the tube labeled  -1. Vortex the tube for 10 seconds to mix the sample.
5. Add 10uL from the tube labeled -1 to the -2 tube. Vortex the tube for 10 seconds.
6. Repeat step 5 for the rest of the tubes by adding 10uL from the previous solution into the next tube. Then vortex.
7. Place 5uL of each sample onto a concavity slide.
8. Place the slide under a microscope and observe.
9. Observe and count how many cells are in each dilution sample. Record observations.

Results:

Conclusion: Overall, students that participated in this lab were able to learn more about serial dilutions. I personally thought that this lab was really interesting to do because we got to see how cells react when living in the same samples, but different dilutions.