Purpose: To run our gel through the UV light machine to potentially find DNA

Procedure:

1. Place the gel holder into the gel electrophoresis chamber. Add 10 ul of ladder (G bioscience 100pb DNA Mark) in the first well. In the next 6 wells, micropipette 5 ul of the negative control, 5 ul of the positive control, and 5 ul of the eDNA and repeat for the second group.
2. Once the PCR solutions are inserted into the gel, place the top on the chamber and connect the red and black wires accordingly. Turn on the box and set it at 100V for 30 minutes.
3. Carefully remove the gel from the chamber and place on a plate to be put into the UV light machine. The BioRad Gel Doc EZ System was used to scan the DNA in our gels

Results/Conclusion:

Due to our concentration of DNA, it was not necessary to dilute our sample by any means. Many groups had a problem with the dispersal of ethidium bromide throughout gel making it more difficult to locate the bands. Our gel turned out very successful. There is a band located next to the V4 region (about 400-500bp) in our DNA and positive control, which is what we wanted. In our negative control, however, there seemed to have been an excess of primer added to the sample, which explains bright band located towards the bottom of the gel. Our soil sample was collected at 31.3157N 96.5144W.