April
27
Madison Powell Lab Day 4/19/17
Madison Powell
4/19/17
Rationale of today’s work: Today Josh, Haley, and I will enter all of our data into excel, and find the consensus sequences.
Tools: Google sheets
Results: We inserted all of the data into a google sheet. Then I went onto find the consensus sequences. Josh worked with the terminator prediction software. Haley did the intro and conclusion. We wrote our abstract and started to put our presentation together.
Our consensus sequences can be seen below:
| AQ | 1 | 2 | 3 | 4 | 5 | 6 |
| Gene 10/11 -10 Promoter | C/A | A | T | A | T/A | T |
| Gene 83/84 -10 Promoter | T | A | T | A | A/T | T |
| -10 Promoter
Consensus |
T | A | T | A | A/T | T |
| Gene 10/11 -35 Promoter | T/G | T | G | A | A/G | A |
| Gene 83/84 -35 Promoter | T | T | G | A/C | C/T/A | A |
| -35 Promoter Consensus | T | T | G | A | A/C | A |
| AQ | 1 | 2 | 3 | 4 | 5 | 6 |
| -10 CONSENSUS | T | A | T | A | A/T | T |
| -35 CONSENSUS | T | T | G | A | A/C | A |
| AN | 1 | 2 | 3 | 4 | 5 | 6 |
| -10 CONSENSUS | T | T | G | A | T | T |
| -35 CONSENSUS | T | T | G | A | C | G |
| E. coli | 1 | 2 | 3 | 4 | 5 | 6 |
| -10 CONSENSUS | T | A | T | A | A | T |
| -35 CONSENSUS | T | T | G | A | C | A |
Conclusion: I was able to compute all of the consensus sequences and we found that the consensus for AQ is similar to that of the sigma 70 for E. coli. Next lab, we will finish putting our presentation together.