April 29

4/19 Data Collection and Gepard Dot Plots

4/19/17

Goal:

Generate a document that compiles all selected amino acid sequences. Generate a Gepard dot plot.

Notes:

We found 3 other proteins (along with tape measure proteins) that were present in Nubia, Caterpillar and Shrooms: Capsid Maturation Protein, Portal Protein, and Terminase Large Subunit.

For each protein we found 3 phages (including one from our class) that had that protein. We copied the amino acid sequence of the gene that coded for the protein and compiled them into one large document.

EX: Capsid Maturation Protease

Caterpillar14_AU (Amino acid sequence)

CapnMurica13_AU (Amino acid sequence)

Gordon13_AU (Amino acid sequence)

Nubia7_AK (Amino acid sequence)

HunterDalle4_AK (Amino acid sequence)

Joann7_AK (Amino acid sequence)

Shrooms16_AL (Amino acid sequence)

Laroye17_AL (Amino acid sequence)

Salgado17_AL (Amino acid sequence)

We compiled a document like this for each protein. We then inputted each document into Gepard and created 4 dotplots.


Posted April 29, 2017 by andrea_springman in category Andrea's Notebook

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