April 25

February 20, 2017 to March 13, 2017: Peer Review of Genes 33-48 and Final DNA Master Upload

Alex Munoz

Lab Day 5: 2/20/17 through Lab Day 8: 3/1/17

Objectives of those lab days: Revising genes 33-48

Tools: DNA Master, Glimmer, GeneMark, NCBI BLAST, PhagesDB BLAST, Starterator, Phamerator

Pranav and I peer reviewed genes 33-48, which were annotated by Christina and Navya. Pranav reviewed the even numbers and I reviewed the odd numbers. Genes 46 and 48 were deleted and all of the entries were put in correctly as far as we could tell. There were a couple of typos in the Master Excel Sheet, but as we reviewed we corrected them.

Results: There were no major corrections that I needed to make, only a few typos.

Next Class: Prepare the Final DNA Master Upload to be revised by Lathan and Dr. Adair.

 

Lab Day 9: 3/13/17

Objective of the day:Preparing Final DNA Master Upload with all new changes/revisions included; get in groups to work on URSA poster

Tools: DNA Master, Glimmer, GeneMark, NCBI BLAST, PhagesDB BLAST, Starterator, Phamerator

I began preparing the Final DNA Master Upload with all of the deletions, insertions, and revisions included. I followed a procedure given by PhagesDB step-by-step and was able to complete the final annotation of Nubia. Dr. Adair also assigned a poster “competition” in which we got into groups of four and create posters to be selected for URSA. I decided to be in a group with Cori, Niru and Noah.

Results: Completed final annotation of Nubia including all revisions and chose a group for my poster.

Next Class: Begin working on poster for URSA

 


Posted April 25, 2017 by alex_munoz in category Alex's Notebook, Lab Notebook

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