Taylor Hutcheson

Date:
17 February 2017

Title:
Evaluating the Experiment

Goals:

• Complete the one week cells counts and serial dilutions we began one week prior to this lab.
• Become familiar with the use of Methyl green-pytonin staining using a wet mount.
• Compare date between the 24-hour cell counts and the 1-week cell counts, as well as its relation to cell groups that were and were not treated with pesticide.

Procedures:

1. Retreive the cultures and observe under a dissecting microscope to see if any cells are still alive.
2. Transfer 2 uL from the control and treatment wells to a concavity slide and observe under a compound microscope so as to count the number of living cells in each.
3. Dilutions may be necessary if there are too many cells to count.

Observations & Data Analysis:

• C1 – control, 24 hour – 285 cells
• C2 – control, 24 hour – 145 cells
• C3 – control, 24 hour – 35 cells
• C4 – control, 7 days – 160 cells
• C5 – control, 7 days – 265 cells
• C6 – control, 7 days – 155 cells
• D1 – treatment, 24 hour – 105 cells
• D2 – treatment, 24 hour – 160 cells
• D3 – treatment, 24 hour – 1115 cells
• D4 – treatment, 7 days – 145 cells
• D5 – treatment, 7 days – 315 cells
• D6 – treatment, 7 days – 180 cells

Next Steps:
The next steps would be to create a graph so as to portray all of the data from my group.