DNA!
After verifying I had a surprisingly accurate calculation for my webbed plate I moved on to flooding and filtering. Unfortunately filters did not quite agree with the gooey arthrobacter(centrifuging it didn’t help either), which just meant I got to spend more time in the lab. Two other students and myself moved on to preparing our samples for DNA extraction. Although I spent an extra large amount of time in the lab, it all payed off on Wednesday when all three of us were able to extract the DNA from our phages and verify its presence and purity. 🙂
Connections
Everything is connected in some way. It is interesting when science can find ways to illustrate these connections. The past and the present are very much a part of each other and the similarities can be exciting.
http://www.the-scientist.com//?articles.view/articleNo/37152/title/A-Bone-Deep-Kinship/
Crispr DNA Sequence
Comment on Two are better than one… by carterlantz
It is so much fun to work with other people. Jeremy, Walker, and I are on different steps, but we still help each other out if we can. It is great to see others succeed, and know that you are going to be on that step soon.
Comment on Some Phage Today by carterlantz
I actually used Msmeg too. I tried to find Arthrobacter and used over 10 samples to find it, but I never found a phage. So I went with Msmeg.
Webbed Plate
once again, going back
Turns out, it was too weak and I lost it again. Going back a few more weeks to try to revive this poor little phage of mine.
Cow’s Milk Cures Cancer?
Flooding
On Wednesday, I got the go ahead to start flooding my plates as my -1 sample’s plaques seemed similar and web-like. I added phage buffer to that plate and put it in the cold room so I will start working with that on Monday.
Imagery
This is now my mental image of what happens when my phage meets the M. Smeg on the plates… http://d3j5vwomefv46c.cloudfront.net/photos/large/820310976.gif
Comment on Two are better than one… by jadeconnor
Awww! Thanks Abby! I am so thankful I have you too. It makes it a lot easier when there is someone else there to check and make sure you are doing things right. This lab would definitely be a struggle without you and I on the same page.
Empirical Plaque Assay for My Phage Lysate
This thing is simply too weak.
Two are better than one…
When most people think about scientific research, they envision a mad scientist working for days without seeing another human being. At least that is how I saw scientific research! However, through this lab I have discovered just the opposite. Now, one could attempt to do everything alone…and it would probably work. However, it would be ten times harder. Jade and I are on the same step in this purification process, and it makes the whole process more enjoyable. In fact, I am learning even more about what I am doing, because I can always compare my results with her results, and see how our phages differ. It is truly exciting to be able to work through an experiment like this with someone else.
Comment on Finally back! by carterlantz
Next Procedure After Spot Test
Finally back!
After being MIA for about a week (I actually missed not being in lab!), I finally got to check out my plates from the week before. Everything seemed to look fine, I got really nice looking plaques and yesterday I was able to do my third and last plaque assay (hopefully) for my purification process. I’m going to check out my plates tomorrow and hopefully I can go onto the next step.
Comment on Robots=Humans? by jadeconnor
I think this area of science is so interesting! I don’t know a lot about what it entails to make such a robot, but I know that they are trying to develop a similar type of robot to be used in the medical field. The idea is that every doctor would get this robot where they could enter in symptoms and facts about the patient and the robot would scan through all the medical records and science article regarding the symptoms and make a recommendation for the course of treatment for the patient. I think once it is fully functional, it would be a great asset to physicians who are constantly pressed for time. Hopefully, though, physicians will still be adept and not rely solely on the robot to make decisions.
Calculation Time
Comment on Don’t Flood On My Plate by Saiaparna_Konde
Oh my gosh this is brilliant and hilarious!! Hahah
Update!
I guess math occasionally has its interesting moments right?…
Well maybe not.. but regardless that was the story of today’s lab: Math. I carefully took my titer count and made my table for my webbing dilutions.
So I feel like in this lab, finding the phage is the obvious first checkpoint, and I feel like the next is getting your calculations and product of 10 webbed plates. So now that I’m getting closer to that second checkpoint, i’m getting excited!
Math, Math, and More Math!!!
On Wednesday in lab I spent the entire class coming up with the calculations for what concentration of phages I would need to make my webbed plate. After many attempts and crossed out numbers, I came up with 14 as my ideal number! According to my calculations I need 14 micro-liters of the 10(6) dilution to make my webbed plate. I am also plating 28 and 56 micro-liters of 10(6) and 7 and 3.5 micro-liters of 10(7) incase my math was slightly off. I am hoping, praying, and crossing my fingers that I calculated correctly and I will have a perfectly webbed plate on Wednesday! Wish me luck! 🙂