Timothy Tatéossian – September 13

– Lab complete September 6

In this lab, we observed last week’s ciliates. The ciliates were kept in relatively dark conditions with minimal food. My observations of the ciliates are:

Well A: Alive

Well B: Unresponsive – Body exists without movement

Well C: Dead

Well D: Dead

Well E: Exactly 2 ciliates were alive

In addition, I created a new well with the model organism Tetrahymena.

Well T: Alive, maybe 1000+. The organisms have a round shape and are almost transparent. There was a noticeably higher concentration of Tetrahymena near the center of the well.

In all wells except well T, I added a drop of Cerophyll to the wells. Since cerophyll could be described as “ciliate fertilizer”, then I expect either the population to grow or that the existing ciliates become more active.

EXPERIMENT DESIGN:

While we did not finalize the experimental design, we did agree on a few things:

• Use Tetrahymena
• Use salt stress
• Measure this by number of organisms in wells
• Use NaCl as stress

We were still finalizing whether to vary the salt or the concentration of one salt.