February 16, 2017

Hypothesis:

The purpose of Lab 6 was to continue to practice serial dilutions and to make the 168-hour and 24-hour cell counts in order to observe how our samples reacted to the (Glycophosate) treatment group and the control group. of later analyze this data and use in our respective research papers.

Background:

Continuing what the experiment started last time, most of fellow lab partners came into to do 24-hour counts. Unfortunately, I had a prior engagement and could not come in to open-lab after 24-hours. So, one of my Lab TA helped me by taking out 2 uL from the the treatment and control group and storing it in the freezer for me to come back and count my samples one week later.

Procedure:

24-Hour Count:

1. Using a micropipette, transfer 450 uL from the 24-hour wells into the microfuge tubes.
2. Label the tubes with your section, initials, date, and well.
3. Proceed to transfer 2 uL from each microfuge tube onto a concavity slide in order to observe it under a compound microscope.
4. View the sample under microscope and then make cell counts (x2).

168-Hour Count:

1. Observe the Tetrahymena under a dissecting microscope.
2. Using a micropipette to transfer 2 uL from the well onto a concavity slide.
3. View this sample under the microscope and make cell counts.

Observations

24-Hour Count

• Row C: Control
  • C1:78
  • C2: 65
  • C3: 120
• Row D: Treatment
  • D1: 140
  • D2: 180
  • D3: 120
• Class Mean=77.125

Average 168-Hour Count

• Row C: Control
  • C1: 1
  • C2: 2.5
  • C3: 9
• Row D: Treatment
  • D1: 100
  • D2: 22.5
  • D3:59.5